摘要
Abstract
To study the significance of catgut embedding at acupoints in reducing body weight and lipid by observing its effect on ingestion and lipid metabolism in rats with simple obesity. Methods A simple obesity model of rats was established by feeding rats with high fat and glucose diet for 10 weeks. Twenty-four SD rats with obesity were randomly divided into control group, model group and catgut embedding(CE) group, 12 rats in each group. Rats in CE group were treated with embedded absorbable catgut at bilateral acupoints of Housanli, Tianshu, and Pishu, once a week. Rats in the other two groups were not interfered. Fifteen days after treatment, body weight and daily ingestion, serum levels of total cholesterol(TC) and low-density lipoprotein cholesterol(LDL-C), activities of lipoprotein lipase(LPL) and hepatic lipase(HL) were measured, respectively. Serum obestatin was assayed by radioimmunoassay and positive expression of obestatin in hypothalamus of rats was detected with immunohistochemical method. Results The serum TC and LDL-C levels were significantly higher while the activities of LPL and HL were significantly lower in model group than in control group(P<0.01). The body weight was decreased greater, the daily food ingestion was less, the serum TC and LDL-C levels were significantly lower, and the activities of LPL and HL were significantly higher in CE group than in model group(P<0.01). However, the serum obestatin level was higher in model group than in control group(P<0.05) but with no difference between model and CE groups. The expression level of obestatin in hypothalamus of rats was higher in model and CE groups than in control group. However, the expression level of obestatin in hypothalamus of rats was significantly higher in CE group than in model group(P<0.01). Conclusion Catgut embedding at acupoints can reduce body weight and lipid in rats with simple obesity by increasing the expression of obestatin in hypothalamus and serum of rats, thus inhibiting food ingestion, strengthening activities of LPL and HL in liver to accelerate serum lipid metabolism and decreasing serum TC and LDL-C levels.关键词
穴位埋线/单纯性肥胖/肥胖抑制素/脂代谢Key words
Acupoint Catgut Embedding/Simple Obesity/Obestatin/Lipid Metabolism分类
中医学
