昆虫学报2011，Vol.54Issue(5)：555-565,11.
大卫绢蛱蝶线粒体基因组全序列测定和分析
Sequencing and analysis of the complete mitochondrial genome of Calinaga davidis Oberthüir ( Leoidootera: Nvmohalidae)
摘要
Abstract
Up to now, the reports about the complete mitochondrial genome of bufterflies and their corresponding molecular evolutions are still limited. In this paper, the complete mitochondrial genome (mitogenome) of Calinaga davidis was sequenced and analyzed using the long PCR and the conserved primer walking technology. The results showed that the entire mitochondrial genome sequence was 15 267 bp long (GenBank accession no. HQ658143), containing 13 protein-coding genes (ATP6, ATP8, COIⅢ, ND1 -6, ND4L and Cytb), 22 transfer RNA genes, 2 ribosomal RNA genes (IrRNA and srRNA) and a putative control region (D-loop). All the 37 genes are arranged in the same orientations as those of other lepidopteran species determined; and there are 11 intergenic spacer sequences totalling 130 bp ( 1 bp to 46 bp for each sequence) and 13 overlapping sequences totalling 66 bp ( 1 bp to 35 bp for each sequence),interspersed throughout the genome, and the largest (46 bp ) spacer region and overlapping region are located between the tRNAGln and ND2, and between COIl and tRNALys genes, respectively. The large and small rRNA genes are 1 337 bp and 773 bp in size, respectively. All the tRNA genes have typical leaf clover secondary structures, except for the tRNASer(ACN), whose DHU arm forms a simple loop. The 13 protein-coding genes are 11 247 bp in length with corresponding 3 737 codons, their base compositions have a relatively higher AT bias and their codon usages are also predominantly A + T-rich. All the protein coding genes (PCGs) use standard initiation codons ATN, except for the COl gene, which uses TTG as its starting codon, and all the PCGs use common stop eodon (TAA), except for the COl and ND4 genes, which terminate into a single T and TA, respectively. The A + T-rich region is 389 bp in length with the AT content up to 92.0％, and this region contains two microsatellite-like repeating sequences (TA)6 and (AAT)4. The results of this study provide some important molecular data to clarify the systematical status of Calinaginae, as well as its phylogenetic relationship with other nymphalid subfamilies.关键词
鳞翅目/大卫绢蛱蝶/线粒体基因组/tRNA二级结构/串联重复序列Key words
Lepidoptera/ Calinaga davidis/ mitochondrial genome/ tRNA secondary structure/ tandemly retpeated seouences分类
生物科学引用本文复制引用
夏靖,胡静,朱国萍,朱朝东,郝家胜..大卫绢蛱蝶线粒体基因组全序列测定和分析[J].昆虫学报,2011,54(5):555-565,11.基金项目
安徽省高校省级自然科学研究重点项目(KJ2010A142) （KJ2010A142）
安徽省优秀青年科技基金(08040106811) （08040106811）
安徽省"重要生物资源保护与利用"重点实验室及中青年学术与技术带头人专项基金(590620) （590620）
北京市自然科学基金重点项目(6081002) （6081002）
