南京农业大学学报2011，Vol.34Issue(3)：1-6,6.
4个两系杂交稻亲本的SSR多态性分析
Analysis of SSR polymorphism among parents of four two-line hybrids in rice( Oryza sativa L.)
摘要
Abstract
Sterility of female parent in two-line hybrid rice is often influenced by the illumination time and temperature.It results in selfing in sterile lines and reduces the quality of hybrid seed.In this study, DNA polymorphism of five parents of four two-line hybrid rice with Peiai64S as female parent were analyzed by 52 pairs of SSR primers with one japonica hybrid rice named 86you8 as the control.46 SSR primers could amplify polymorphism among 7 parents.8 pairs of SSR primers which could amplify polymorphism among the parents of 86you8 could distinguish the 4 two-line combinations from the control.There were 34,32,31,30 and 14 pairs of SSR primers which could be used to distinguish the combinations (F1)and the parents of Liangyoupeijiu, Liangyoul08,Peiai64S/E32,Liangyou122 and 86you8, respectively.16 SSR primers could distinguish all of the 4 two-line hybrid rice and their parents.RM206 and RM286 could distinguish the five combinations and their parents in this study.RM505 was tested to identify Liangyoupeijiu and its parents.The result showed that it could distinguish Liangyoupeijiu and its parents accurately.There were several kinds of methods to distinguish the 4 two-line combinations according to SSR polymorphism and specificity in this study.The four two-line combinations of Liangyoupeijiu, Liangyou108, Peiai64S/E32 and Liangyoul22 could be distinguished by the group of four pairs of primers including RM13 ( or RM206, RM286 and so on), RM224 ( or RM337 ), RM234 ( or RM252, RM505 and RM565 )and RM25 ( or RM217, RM248 and RM585).关键词
两系杂交稻/SSR标记/多态性Key words
two-line hybrid rice/SSR marker/polymorphism分类
农业科技引用本文复制引用
戴剑,洪德林,吴燕,陈兰..4个两系杂交稻亲本的SSR多态性分析[J].南京农业大学学报,2011,34(3):1-6,6.基金项目
国家863计划项目(2010AA101301) （2010AA101301）
江苏省农业科学院科研基金项目(6110830) （6110830）
