畜牧兽医学报2011，Vol.42Issue(5)：679-684,6.
马流感病毒双抗体夹心ELISA检测方法的建立
Development of Double Antibody Sandwich ELISA for Detection of Equine Influenza Virus
摘要
Abstract
To develop a rapid and effective method for Equine influenza virus (EIV) detection,polyclonal antibodies against EIV A/equine/Xinjiang/07 strain and monoclonal antibody against NP of EIV were generated respectively. Then a double antibody sandwich ELISA (DAS-ELISA)was developed. The specificity of the optimized DAS-ELISA was evaluated using EIV, Equine arteritis virus, Equine herpes virus-1, Equine herpes virus-4 and Japanese encephalitis virus, resulting in only EIV specimens yielding a strong signal. Compared with hemagglutination test, its sensitivity was as two point five to ten times as the later. And it had cross-reactivity with H7N7 subtype. Meanwhile it is suitable for detection of virus from the nasal swabs of experimentally infected equines. The results revealed that the ELISA possessed good specificity and higher sensitivity, indicating a suitable method for rapid detection of EIV.关键词
马流感病毒/多克隆抗体/单克隆抗体/双抗体夹心ELISA分类
农业科技引用本文复制引用
姬媛媛,郭巍,王晓钧,王征,卢刚,赵立平,李红梅,相文华..马流感病毒双抗体夹心ELISA检测方法的建立[J].畜牧兽医学报,2011,42(5):679-684,6.基金项目
黑龙江省自然科学基金重点项目(ZD200812) （ZD200812）
