畜牧兽医学报2011，Vol.42Issue(5)：685-691,7.
鸭源H5亚型禽流感全禽源分子标记疫苗候选株的构建
Generation of Duck Origin H5 Subtype Avian Influenza Vaccine Candidate with Heterogenous NA Gene
摘要
Abstract
The aim of this study was to develop H5 subtype avian influenza vaccine with all genes from avian origin influenza viruses, and provide a DIVA ( Differentiating Infected from Vaccinated Animals) strategy for the control of avian influenza. Sequences encode several basic amino acids of HA gene from A/mallard/Huadong/S/2005(H5N1,S strain) were deleted. Using reverse genetics system, we rescued two reassortant viruses (rH5N6/F and rH5N2/F) that contained NA genes from A/duck/England/1/1956 ( H11N6, E strain) or A/Chicken/Shanghai/F/98( H9N2, F strain) and internal genes from F strain The two vaccine viruses were avirulent to SPF chickens and embryonated chicken eggs, replicating well in eggs and MDCK cells, while rH5N6/F showed better growth in eggs. The results indicate that the reassortant viruses are suitable for vaccine manufacturing and being used as reference vaccine virus against the H5NI clade 2. 3. 4 viruses in China.关键词
禽流感病毒/反向遗传/H5/DIVA疫苗分类
农业科技引用本文复制引用
张文俊,薛涛,吴小伟,唐应华,彭大新,刘秀梵..鸭源H5亚型禽流感全禽源分子标记疫苗候选株的构建[J].畜牧兽医学报,2011,42(5):685-691,7.基金项目
现代农业产业技术体系建设专项资金(nycytx-41-G07) （nycytx-41-G07）
国家重点基础研究发展计划(973计划)(2011CB505003) （973计划）
