天然产物研究与开发2011，Vol.23Issue(2)：275-278,4.
大孔吸附树脂对苜蓿皂苷的吸附和解吸附作用
Adsorption and Desorption of Alfalfa (Medicago sativa L. ) Saponins by Macroporous Resin
摘要
Abstract
Five types of macroporous adsorption resins namely AB-8, S-8, NKA-9, D-101 and X-5 with varying polarity were evaluated for the adsorption and desorption of alfalfa saponins. Of these,the moderately polar AB-8 resin showed the highest adsorption capacity and the absorbed saponius could be eluted effectively with 55-65％ aqueous ethanol. The adsorption reached saturation at 1: 1 alfalfa extract to AB-8 resin mass ratio. The maximum desorption capacity of alfalfa extract was 108.4 mg/g dw AB-8 resin on a column eluted by 90％ aqueous ethanol. The saponin content in the concentrated 90％ aqueous ethanol effluent reached 53％ ,about 9 times of that (5.68％) in the crude alfalfa extract. The AB8 maeroporous resin has may be a favorable sorbent for large-scale saponin isolation from alfalfa.关键词
紫花苜蓿/皂苷/大孔吸附树脂/吸附/解吸附Key words
Medieago safina/ saponins/ macroporous resin/ adsorption/ desorption分类
农业科技引用本文复制引用
王蓟花,周雨凡,贺伟,韩建国,玉柱,周立刚..大孔吸附树脂对苜蓿皂苷的吸附和解吸附作用[J].天然产物研究与开发,2011,23(2):275-278,4.基金项目
This work was co-financed by the grants from the Commonweal Fund of China Agriculture(nyhyzx07-022),the National Key Technology R&D Program of China(2006BAD16B08),and the Introdueing International Advanced Agricultrual Project of the Ministry of Agricultural Project of the Ministry of Agriculture of China(2006-G38). （nyhyzx07-022）
