天然产物研究与开发2011，Vol.23Issue(2)：295-298,294,5.
胰蛋白酶水解β-乳球蛋白获得ACE抑制肽的条件优化
Optimizing Hydrolysis Conditions of β-Lactoglobulin with Trypsin to Obtain the ACE Inhibitory Peptide
摘要
Abstract
Three-factor quadratic orthogonal rotation design and in vitro assay was adopted to optimize the hydrolysis conditions of β-lactoglobulin with trypsin for obtaining the Angiotensin-I-converting enzyme (ACE) inhibitory peptide. The results showed that the regression equation of the impacts of the substrate concentration ( X1 ), temperature ( X2 ), enzyme ,and substrate mass ratio ( X3 ) on the ACE inhibition rate was Y = 50.62-2.33X1 -1.97X2 + 5.81 X3-3.36X2X3-6.56X22-1.96X32. The optimum conditions were as following:when substrate concentration was at 60 g/L,the hydrolysis temperature at 30 ℃ ,enzyme and substrate mass ratio at 5.5％ ,the hydrolysis time in 6 h,the hydrolysis product of ACE inhibitory activity was on the maximal inhibition rate of 53.86％.关键词
β-乳球蛋白/胰蛋白酶/ACEKey words
β-lactoglobulin/ trypsin/ ACE分类
轻工纺织引用本文复制引用
梅林,王志耕,王子龙,薛秀恒..胰蛋白酶水解β-乳球蛋白获得ACE抑制肽的条件优化[J].天然产物研究与开发,2011,23(2):295-298,294,5.基金项目
科技部科技人员服务企业行动项目(2009JGC30016) （2009JGC30016）
