重庆医学2011，Vol.40Issue(17)：1733-1735,封3,4.DOI:10.3969/j.issn.1671-8348.2011.17.028
低温冻存对人绒毛膜来源的间充质干细胞多向分化潜能的影响
Influence of cryopreservation on multilineage differentiation potential of human chorion-derived mesenchymal stem cells
陈霞 1尹晓娟2
作者信息
- 1. 中国人民解放军第三二四医院儿科,重庆,400020
- 2. 中国人民解放军北京军区总医院附属八一儿童医院新生儿科,北京100700
- 折叠
摘要
Abstract
Objective To explore the influence of cryopreservation on growth characteristics and multilineage differentiation potential of human chorion-derived mesenchymal stem cells (hCDMSC) in vitro and verify the feasibility of cryopreserved hCDMSC serving as seed cells for tissue engineering.Methods Collagenase and trypsin were used to trypsinize placental basal decidua,cells were subcultured and the fourth generation cells were resuscitated after 3 months cyropreservation in liquid nitrogen under the conditions of deep hypothermia( -196℃ ).MTT method was employed to measure the cellular proliferation of resuscitated hCDMSC and alizarin red and oil red O staining were applied to detect the osteogenic and adipogenic differentiation.hCDMSC without cyropreservation served as a control.Results There were no significant difference in proliferation activity and multilineage differentiation potential of resuscitated hCDMSC after cryopreservation compared to those without cryopreservation.hCDMSCs demonstrated positive staining with aizarin red and oil red O.Conclusion Cryopreservation has no influence on proliferation and multilineage differentiation potential of hCDMSC in vitro ,and cryopreserved hCDMSCs can serve as seeds for tissue engineering.关键词
间质干细胞/绒毛膜绒毛取样/低温保存/细胞分化/细胞增殖Key words
mesenchymal stem cells / chorionic villi sampling / cryopreservation / cell differentiation / cell proliferation引用本文复制引用
陈霞,尹晓娟..低温冻存对人绒毛膜来源的间充质干细胞多向分化潜能的影响[J].重庆医学,2011,40(17):1733-1735,封3,4.
