安徽农业科学2011，Vol.39Issue(12)：6953-6955,6958,4.
1株乳酸生产菌的筛选及发酵培养基的优化
Screen of the Bacterial Strains for Lacti Acid Production and Optimization of its Fermentation Medium
崔东波 1苑广志1
作者信息
- 1. 辽宁农业职业技术学院,辽宁营口,115009
- 折叠
摘要
Abstract
[ Objective ] The basis of the lactic acid fermentation was laid. [ Method The bacterial strain LP3-3, which had a higher yield of lactic acid, was obtained from 14 alkali-resistant microorganisms. The medium for the LP3-3 fermentation was optimized with single-factor experiment. [ Results] The results indicated that the LP3-3 was confirmed to be Exiguobacterium aurantiacum C/N based.on the preliminary identification of 16S rRNA sequencing. The optimal experiment in its fermentation medium indicated the glucose was used as the source of carbon,the yeast extract with the amount of 1％ was used as nitrogen and the ratio of C/N was 5∶ 1. And the best component in medium was the glucose was 50.0 g/L,the yeast extract was 10.0 g/L,the peptone was 4.0 g/L,the sodium acetate( anhydrous)2.0 g/L,the ammonium citrate was 2.0 g/L, K2HPO4 · H2O was 2.0 g/L,MgSO4 · 7H2O was 0.2 g/L,MnCl2 · 4H2O was 0.05 g/L,the Twain 80 was 1 ml/L,NaCl was 40 g/L and the initial pH value was 9. 0. [ Conclusion] The activity of LP3-3 in lactic acid fermentation was raised in the optimal medium.关键词
乳酸生产菌/筛选/发酵培养基Key words
Bacterial strain producing lacti acid/ Screen/ Fermentation medium分类
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崔东波,苑广志..1株乳酸生产菌的筛选及发酵培养基的优化[J].安徽农业科学,2011,39(12):6953-6955,6958,4.
