安徽农业科学2011,Vol.39Issue(12):7040-7042,3.
多黏芽孢杆菌液态发酵条件的初步研究
Study on Liquid Fermentation Culture Conditions for P. polymyxa
摘要
Abstract
[ Objective ] The aim was to optimize the liquid fermentation culture conditioas for P. polymyxa. [ Method ] By single test and orthogonal test, the liquid-state fermentation culture conditions for P. polymyxa were determined. [ Result ] The optimum medium component and culture conditions for P. polymyxa were as follow: Potato250.0 g/L, sugar 20.0 g/L, com flour 30.0 g/L, NaNO3 1.0g/L, ( NH4 ) 2 SO4 1.5 g/L, KH2PO4 1.0 g/L,pH6.5, culture medium amount 100 mL/250 mi (V/V) flask, inoculum 5% ( V/V), culture temperature 37 ℃,shake rotation 150 r/min, fermentati-on period 72 h. [ Conclusion ] The study can provide reference for industrial production of P. polymyxa.关键词
多黏芽孢杆菌/发酵/培养基Key words
P. polymyxa / Fermentation/ Medium分类
农业科技引用本文复制引用
胡飞,周子燕,李昌春,胡本进,徐丽娜..多黏芽孢杆菌液态发酵条件的初步研究[J].安徽农业科学,2011,39(12):7040-7042,3.基金项目
国家成果转化项目(2010GB2C300196). (2010GB2C300196)