中国当代儿科杂志2011，Vol.13Issue(4)：273-277,5.
儿童紫癜性肾炎肾组织Th1/Th2比值与肾脏微血管损伤的关系
Relationship between renal Th1/Th2 ratio and renal microvascular injury in children with Henoch-Sch(o)nlein purpura nephritis
摘要
Abstract
Objective To explore possible correlations between renal Th1/Th2 ratio and renal microvascular injury in children with Henoch-Sch(o)nlein purpura nephritis (HSPN).Methods Thirty-two children with HSPN were enrolled.They were classified into four groups by renal pathology: HSPN class Ⅱ ( n = 8 ), HSPN class Ⅲa ( n = 7 ), HSPN class Ⅲb (n = 10) and HSPN class Ⅳ/Ⅴ (n = 7).Five patients undergoing nephrectomy due to trauma were used as the controls.INFγ, IL-4 and CD34 in the renal tissues were measured by immunohistochemical analysis.INFγwas used as a marker of Th1, IL-4 was used as a marker of Th2 and CD34 was used as a marker of microvessel.The renal microvessel density was evaluated according to the Weidner standard.The relationships among the local Th1/Th2 ratio, renal pathological grade, microvessel score and microvessel density were studied.Results Immunohistochemical analysis showed a lower expression of INFγand a higher expression of IL-4 in the HSPN groups than in the control group.The local Th1/Th2 ratio in the HSPN groups decreased and correlated significantly with the renal pathological grade.There were significant differences among four HSPN subgroups ( P ＜ 0.05 ).Compared with the control group, the renal microvessel density in the HSPN class Ⅱ and class Ⅲa groups increased significantly ( P ＜ 0.05), but it decreased in the HSPN class Ⅳ/Ⅴ group ( P ＜ 0.05 ).The renal microvessel scores in the HSPN class Ⅲa, class Ⅲb and class Ⅳ/Ⅴ groups increased signifieantly compared with those in the control and the HSPN class Ⅱ.The increased renal microvessel scores were associated with more severe renal pathological changes.A negative correlation was found between the local Th1/Th2 ratio and the microvessel density in kidneys (r = -0.921, P ＜0.01 ).Conclusions The decrease of Th1/Th2 ratio in kidneys might be responsible for renal microvascular injury in children with HSPN.关键词
紫癜性肾炎/Th1/Th2比值/微血管损伤/儿童Key words
Henoch-Sch(o)nlein purpura nephritis/ Th1/Th2 ratio/ Microvascular injury/ Child分类
医药卫生引用本文复制引用
黄丹琳,许自川,党西强,曾雪琪,何小解,易著文,何庆南..儿童紫癜性肾炎肾组织Th1/Th2比值与肾脏微血管损伤的关系[J].中国当代儿科杂志,2011,13(4):273-277,5.基金项目
国家自然科学基金项目(编号30500546) （编号30500546）
湖南省科技厅科技计划项目(编号06SK-3029-11). （编号06SK-3029-11）
