南京农业大学学报2011，Vol.34Issue(3)：112-116,5.
早期和后期限饲对肉鸡采食量及下丘脑POMC、NPY和瘦素受体mRNA转录的影响
Effect of early and late feed restriction on feed intake and hypothalamic NPY, POMC and leptin receptor gene transcription in broilers
摘要
Abstract
Newly hatched broiler chicks were allocated randomly into control and early feed restriction( ER)groups and later feed restriction(LR) groups. The ER chickens were deprived of food on alternate days for the first 2 weeks from post hatch,and followed by ad libitum feeding for the subsequent 7 weeks,and LR chickens followed the same procedure from 7 to 9 weeks old. During the whole investigation periods,body weight,average daily feed intake and the ratio of feed to gain were escalated. 10 chickens from each group were sacrificed on 2 and 9 weeks old, respectively, and the hypothalamus tissues were taken for pro-opiomelanocortin ( POMC), neuropeptide Y(NPY) and leptin receptor mRNA expression by relative RT-PCR using 18S rRNA as an internal-standard. The body weight of ER group chickens exhibited significantly lower compared with their control and LR counterparts during the whole experimental periods. Compared with control chickens,the body weight of LR chickens at the first week of food restriction decreased significantly,however, there was no difference at the end of the second week food restriction. Average daily food intake was continuously lower in ER group than that of control group from 1- to 8-week-old, on the contrary, which was much higher in LR group during the 2 weeks food restriction. The ratio of feed to gain increased in ER and LR groups during the feed restriction. RT-PCR results showed that, the mRNA expression of POMC, NPY and leptin receptor in the hypothalamus showed an age-dependent increase,and a significant down-regulation of hypothalamic POMC gene expression was observed in ER but not in LR group compared with control group,whereas no significant differences of POMC,NPY and leptin receptor mRNA expression in the hypothalamus was detected in LR chickens on 9 weeks old. In this study, the results suggested that early feed restriction retarded feed intake, which may account for the lean body weight during the whole investigation. On the other hand,the stable expression of appetite related genes in the hypothalamus may be related to the adaptation of chickens to the status of feed restriction.关键词
限饲/阿片-促黑素细胞皮质素原(POMC)/神经肽Y(NPY)/leptin受体/下丘脑Key words
feed restriction/ pro-opiomelanocortin(POMC)/ neuropeptide Y ( NPY )/ leptin receptor/ hypothalamus分类
农业科技引用本文复制引用
倪迎冬,袁丽霞,饶开晴,李玥 ,杨晓静,赵茹茜..早期和后期限饲对肉鸡采食量及下丘脑POMC、NPY和瘦素受体mRNA转录的影响[J].南京农业大学学报,2011,34(3):112-116,5.基金项目
国家973计划项目(2004CB117505) （2004CB117505）
