南京农业大学学报2011，Vol.34Issue(3)：131-134,4.
氨基甲酸酯类农药适体筛选技术的建立
Establishment of aptamer screening technique for carbamate pesticide
摘要
Abstract
To obtain the aptamers with high affinity and specificity for carbamate pesticide, a synthesized 91nt single stranded DNA (ssDNA) random library containing 30 random sequences flanked by invariant primer was subjected to 9 rounds of selection against carbamate pesticide according to SELEX protocol. With the structure changing of nucleic acids from DNA-DNA duplex to a DNA-target complex, the specific aptamers could be identified and separated for further, ssDNA library generation from dsDNA with NaOH employed. In our study, a simple method of screening specific aptamer from no modification pesticide molecules was developed, which could be used as a tool for pesticide detection. After 9 rounds selection,the switching activity increased from 0. 70 to 13.32.关键词
适体/指数富集的配基系统进化技术(SELEX)/荧光/氨基甲酸酯类农药Key words
aptamers/ systematic evolution of ligands by exponential enrichment(SELEX) /fluorescence/carbamate pesticide分类
生物科学引用本文复制引用
王耘,贺江,王丽,刘贤进..氨基甲酸酯类农药适体筛选技术的建立[J].南京农业大学学报,2011,34(3):131-134,4.基金项目
江苏省农业科技自主创新资金项目(CX(10)236) （CX(10）
国家自然科学基金项目(30871658) （30871658）
