河南农业科学2011，Vol.40Issue(3)：85-87,3.
槲皮素和硫脲对桑尺蠖多酚氧化酶的抑制作用
Inhibitory Effects of Quercetin and Thiourea on the Polyphenol Oxidase from Phthonandria atrineata
摘要
Abstract
Polyphenol oxidase plays important roles in metamorphosis development and immune defense of insects. In order to provide the theoretical basis for developing new pest inhibitors with the enzyme as a target,the inhibition effects of quercetin and thiourea on polyphenol oxidase extracted from 5th instar larvae of Phthonandria atrineata were studied. The results indicated that quercetin changed Michaelis constant but not maximum reaction rate of the polyphenol oxidase.Thiourea changed maximum reaction rate but not Michaelis constant. Therefore, quercetin and thiourea could be competitive and non-competitive inhibitors for polyphenol oxidase,respectively.关键词
桑尺蠖/多酚氧化酶/槲皮素/硫脲/抑制作用Key words
Phthonandria atrineata/ Polyphenol oxidase/ Quercetin/ Thiourea/ Inhibitory effects分类
农业科技引用本文复制引用
张永亮,朱勇..槲皮素和硫脲对桑尺蠖多酚氧化酶的抑制作用[J].河南农业科学,2011,40(3):85-87,3.基金项目
教育部博士学科点基金(20060635008) （20060635008）
重庆市教委基金(040208) （040208）
