河南农业科学2011，Vol.40Issue(3)：92-95,4.
不同昆虫泛素延伸蛋白基因的生物信息学分析
Bioinformatic Analysis of Ubiquitin Extension Protein Genes from Different Insects
摘要
Abstract
Based on the published sequences of ubiquitin extension protein (UBE) genes of Spodoptera exigu , Pieris rapa , Helicoverpa assulta , Drosophila melanogaste in NCBI database, the genetic diversity and molecular evolution of the nucleotide sequences, as well as structural domains and motif characters of putative amino acid sequences were analyzed using the bioinformatics method. Results showed high identity (81.8％-99.7％) and short genetic distances (0. 003-0. 199)between the four UBE gene sequences, and all encoded ubiquitin/L40 UBE. During the genetic diversity analysis,81 polymorphic sites in total were detected from 4 UBE gene sequences and 4haplotypes were sorted. Abundant genetic diversity and strong codon bias were found based on the haplotype diversity (Hd= 1.000),the average number of nucleotide difference (K=45. 883), the nucleotide diversity (Pi=0. 112 97), the nucleotide diversity of synonmyous sites (Pi(s)=0. 415 01 ), the nucleotide diversity of nonsynonmyous sites (Pi(a)= 0. 02489), the effective number of codons (ENC=38. 070-53. 458＜61),the codon bias index (CBI=0. 437-0. 729＞0) and the scaled Chi-square (X2 =0.454-0. 960). Each amino acid sequence deduced from its corresponding UBE gene contained one ubiquitin homologue and one Ribosomal/L40 domain, respectively, but signal peptide and transmembrane domain were not found. Fourteen motifs were detected among 4 amino acid sequences. Deduced amoino acid sequences from the Se-UBE gene and Dm-UBE gene contained 1 casein kinase Ⅱl phosphorylation site and 2 protein kinase C phosphorylation sites. Deduced amino acid sequences from the Pr-UBE gene and Ha-UBE gene both had 2 casein kinase Ⅱ phosphorylation sites and 2 protein kinase C phosphorylation sites. The result provided some basic information for further studying the evolution and expression characteristics of UBE genes as well as mechanism of insect viral infection.关键词
泛素延伸蛋白/甜菜夜蛾/菜粉蝶/烟夜蛾/黑腹果蝇/生物信息学Key words
Ubiquitin extension protein/ Spodoptera exigu / Pieris rapa / Helicoverpa assulta /Drosophila melanogaster / Bioinformatics分类
生物科学引用本文复制引用
强承魁,王胜永,凤舞剑,于玲雅,秦越华..不同昆虫泛素延伸蛋白基因的生物信息学分析[J].河南农业科学,2011,40(3):92-95,4.基金项目
徐州生物工程职业技术学院院级科研课题(2010B07) （2010B07）
