河南农业科学2011，Vol.40Issue(3)：149-153,5.
鸡传染性法氏囊病病毒河南分离株HeYD VP2基因高变区基因克隆及序列分析
Sequence Analysis of the VP2 Hypervariable Region of A Field Infectious Bursal Disease Virus Strain HeYD Isolated from Henan Province
摘要
Abstract
To identify the subtype of a field infectious bursal disease virus(IBDV) strain HeYD isolatedfrom Henan province, a pair of specific primers were designed according to the genome sequences of IBDV previously reported for amplifying the cDNA of IBDV VP2 hypervariable region.The VP2 hypervariable region of HeYD was cloned by reverse transcription polymerase chain reaction (RT - PCR), sequenced and compared with other reference IBDV strains.The resultsshowed that the amino acid sequence of HeYD VP2 hypervariable region represents the typical feature of those of very virulent IBDV (vvIBDV) strains at the positions of 222 (A), 256 (I), 294(I),and 299 (S).The phylogenetic tree based on the DNA sequences of IBDV VP2 hypervariable regions indicated that HeYD strain was far away from some of China vvIBDV stains,but close to other China vvIBDV strains such as xin - 1, GX8 - 99, and some foreign vvIBDV strains such as OKYM and DV86.关键词
传染性法氏囊病病毒/VP2基因高变区/基因克隆/基因进化树Key words
IBDV/ VP2 gene hypervariable region/ Gene cloning/ Phylogenetic tree分类
农业科技引用本文复制引用
鄂巍,张改平,罗俊,滕蔓,王兴涛,王爱萍..鸡传染性法氏囊病病毒河南分离株HeYD VP2基因高变区基因克隆及序列分析[J].河南农业科学,2011,40(3):149-153,5.基金项目
河南省基础与前沿计划项目(082300433201) （082300433201）
