安徽农业科学2011，Vol.39Issue(5)：2680-2682,3.
SPE-柱前衍生-HPLC法研究阿维菌素在甘蓝及土壤中的残留动态
Research on the Residue Dynamics of Avermectin in Cabbage and Soil with the Method of SPE-Pre-column Derivatization-HPLC
摘要
Abstract
[ Objective ] An analytical method for the determination of avermectin residue in cabbage and soil was developed based on the precolumn derivatization-SPE-HPLC and the residue dynamics and final residue of avermectin in the cabbage and soil were studied. [ Method ] The sample of plant or soil was extracted with acetonitrile and purified by C18 solid-phase extraction (SPE) cartridge, then, constant volume with acetonitrile after its derivatization by N-methylimidasole (NMIM) and trifiuoroacetic anhydride (TFAA), and finally, the content of avermectin residue was determined with RP-HPLC fluorescence detector(λex =365 nm and λem ＝475 nm). [ Result] The results showed that the half-live of avermectin in cabbage and soil was 1. 5 - 5.4 days and 6.7 - 8.8 days, respectively. Thus it was suggested that the suspension agent of 45.5％ avermectin · diafenthiuron could be used for two times in the control of diamondback moth in cabbage of cruciferae and the safe interval period was 4 days. [ Conclusion] The analytical method was simple, which result was met with the requirement of relevant standard in precision, accuracy and sensitivity.关键词
阿维菌素/甘蓝/土壤/残留动态Key words
Avermectin/ Cabbage/ Soil/ Residue dynamics分类
农业科技引用本文复制引用
马婧玮,张军锋,尹海燕,吴绪金,刘继红..SPE-柱前衍生-HPLC法研究阿维菌素在甘蓝及土壤中的残留动态[J].安徽农业科学,2011,39(5):2680-2682,3.基金项目
农业部农药检定所残留项目(2008P254). （2008P254）
