中国畜牧兽医2011，Vol.38Issue(3)：104-107,4.
猪瘟石门系强毒株SYBR Green-I染料法实时荧光定量PCR方法的建立
Development of a Real-time Flourescent Quantitative PCR for Detection of Classical Swine Fever Virus Shimen Strain
摘要
Abstract
In order to be able to fast and accurate diagnosis of classical swine fever virus strain Shimen based on the alignment of genomic sequences of 23 strains of Shimen available in GenBank, one pair of differential primers were designed by DNASIS software. SYBR Green- Ⅰ real-time PCR for detection of classical swine fever virus strain Shimen was established.The sensitivity was 100 copies/μL. The assay is highly specific and showed no cross reactivity against PRRSV, PCV2, PRV,BVDV-1 and C-strain. Inter-assay and intra-assay variables of CV were less than 0.7 ％. This method was highly sensitive specific and rapid. This assay could provide a powerful tool for quantification of Shimen-strain, and could be further developed into a commercial kit for rapid detection of Shimen-strain.关键词
猪瘟病毒/Shimen毒株/SYBR/Green-Ⅰ染料法实时荧光定量PCRKey words
CSFV/ Shimen-strain/ SYBR Green- Ⅰ real-time PCR分类
生物科学引用本文复制引用
王伟,邹月红,李雪松,陈欣,郎越坤,田华斌,符芳,李曦,李集临..猪瘟石门系强毒株SYBR Green-I染料法实时荧光定量PCR方法的建立[J].中国畜牧兽医,2011,38(3):104-107,4.基金项目
黑龙江省科技攻关重点项目(6130513501 1). （6130513501 1）
