中国畜牧兽医2011，Vol.38Issue(3)：146-149,4.
石歧杂鸡催乳素基因外显子4变异与产蛋就巢性状的关联分析
SNP Detection of Exon 4 of Prolactin Gene and its Associations with Egg-laying and Broodiness Traits in Shiqiza Chicken
摘要
Abstract
Exon 4 of prolactin gene were detected by PCR-SSCP and the DNA fragments were sequenced.The correlation analysis between the polymorphism of the exon 4 and egg-laying and broodiness traits were carried out respectively.The results indicated that there were one polymorphic sites in exon 4.A G→A mutation at the position of the 6017 bp was found,which resulted in 3 genotypes(AA,AB and BB) ,but the mutation did not lead to changes in amino acid.The frequencies of genotype AA and allele A were the highest.The least square analysis showed that the first broodiness day and age at 1st egg of BB type chickens was later than AA and AB types chickens,The locus of variation showed a definite trend in the age at 1st egg and the first broodiness day.关键词
PRL基因/PCR-SSCP/石歧杂鸡/就巢性状Key words
PRL gene/ PCR-SSCP/ Shiqiza chicken/ broodiness traits分类
农业科技引用本文复制引用
逯岩,李福伟,曹顶国,雷秋霞,韩海霞,周艳,李桂明,黄保华..石歧杂鸡催乳素基因外显子4变异与产蛋就巢性状的关联分析[J].中国畜牧兽医,2011,38(3):146-149,4.基金项目
国家行业科技计划(3-46、3-26) （3-46、3-26）
山东省良种工程项目(2009LZ014) （2009LZ014）
现代农业产生技术体系建设专项资金资助(nycytx-41-z09) （nycytx-41-z09）
山东省农业科学院创新基金会(2007-ycx013). （2007-ycx013）
