中国畜牧兽医2011，Vol.38Issue(3)：199-203,5.
羊布鲁氏菌强毒株16M感染小鼠巨噬细胞模型的建立
Eestablishment of Model that Virulent Brucella melitensi strain 16M Infect Mouse Macrophages
摘要
Abstract
Macrophage was the strongest anti-body cells of phagocytosis, while, Brucella could multiply in the cell and could not be killed. Therefor,we established the infection model,for continuous research Brucella and it's the host cell,and the interaction of baceria cell and host cell surface-associated membrane protein,discovering the intracellular interaction mechanism for next step. Infection of mouse macrophage cell line RAW264.7 with Brucella melitensi virulent strain 16M were performed 4 h at a cell/bacteria ratio of 1 : 500. Brucella-infected marophages were examined by indirect immunofluorescence assay and transmission electron microscopy assay. The optimal concentration ratio of primary antibody and secondary antibody in indirect immunofluorescence assay were 1 : 80 and 1 : 80, and the membrane depression and the endocytosis bodies were observed by transmission electron microscope. Expriment reduced the environmental factors of the infection process, optimized concentration ratio of primary and secondary antibodies in indirect immunofluorescence assay,established infection model.关键词
羊布鲁氏菌强毒株16M/巨噬细胞系RAW264.7/间接免疫荧光试验/透射电镜试验Key words
virulent Brucella melitensi strain 16M/macrophage cell line RAW264.7/indirect immunofluorescence assay/ electron microscope assay分类
农业科技引用本文复制引用
孙春辉,郎需龙,杨艳玲,王秀然,李晓燕,卜昭阳,王兴龙..羊布鲁氏菌强毒株16M感染小鼠巨噬细胞模型的建立[J].中国畜牧兽医,2011,38(3):199-203,5.基金项目
国家转基因新品种培育重大项目(2009ZX08009163B). （2009ZX08009163B）
