中国美容医学2011，Vol.20Issue(4)：614-617,4.
水蛭素对皮肤增生性瘢痕成纤维细胞bFGF及TGFβ1表达的影响
Effects of Hirudin on expression of bFGF,TGFβ1 in dermal hypertrophic scar fibroblasts
摘要
Abstract
Objective To investigate the influence of Hirudin on the expression of basic fibroblast growth factor (bFGF)and transforming growth factor beta 1 (TGFβ1) in human hypertrophy scar dermal fibroblasts in vitro, therefore to explore the role of bFGF and TGFβ1 on hypertrophy scaring.Methods Human dermal hypertrophy scar derive fibroblasts were obtain through primary culture.Dermal hypertrophy scar fibroblasts were cultured in vitro with Hirudin for 24 hours;mRNA levels of bFGF and TGFβ1 in treated fibroblasts were detected by Realtime RT-PCR, and protein levels of bFGF,TGFβ1 in fibroblasts were detected by immunocytochemistry.Results 0.156～2.5 U/L Hirudin statistically up-regulatedlevel of bFGF mRNA, bFGF (P ＜0.05), and obviously down-regulated level of TGFβ1 mRNA,TGFβ1 (P＜0.05) in hypertrophic scar fibroblasts.Conclusions Hirudin inhibited the secretion of TGFβ1 and facilitated that of bFGF in scar derive fibroblast.bFGF and TGFβ1 could be a regulation target on the growth and proliferation of fibroblasts during scar formation under Hirudin treatment.关键词
瘢痕/水蛭素/成纤维细胞/碱性成纤维细胞生长因子/转化生长因子β1Key words
scar/ Hirudin/ fibroblast/ basic fibroblast growth factor/ transforming growth factor beta 1分类
生物科学引用本文复制引用
郭睿,农晓琳,邓凌,李佳荃,李菊裳,黎燕宁,李昊..水蛭素对皮肤增生性瘢痕成纤维细胞bFGF及TGFβ1表达的影响[J].中国美容医学,2011,20(4):614-617,4.基金项目
国家自然科学基金项目(81060156) （81060156）
广西自然科学基金资助项目(桂科青0447033) （桂科青0447033）
广西中医药科技专项重点课题(GZKZ10-010) （GZKZ10-010）
广西研究生教育创新计划项目(2007重-16,201010598RY10) （2007重-16,201010598RY10）
