工业微生物2011，Vol.41Issue(3)：66-70,5.DOI:10.3969/j.issn.1001-6678.2011.03.013
过氧化氢酶基因在大肠杆菌中的克隆表达及发酵优化
Cloning, expression and fermentation optimization of a catalase gene in E.Coli
摘要
Abstract
The catalase gene katA from Bacillus subtilis WSHDZ-0l was am plified by PCR . It was then inserted into the expmssion vector pET-20b(+ ) to create the recom binant plasmid pET-20b (+ )-katA and them odified vector was transformed into E . coli JM 109 for expression . The size of the expressed specific band by SDS-PAGE electrophoresis corresponded well to that of the catalase gene ka tA . For batch ferm entation in flasks , them edium compositon rpgarding the caroon and nitrogen was optimized and the results indicated that the maxinal enzyme activity of 20000 U /m L was obtained with 5 g/L glycerol and 40 g/L yeast extract, respectwely .关键词
过氧化氢酶/枯草芽孢杆菌/质粒载体/发酵优化Key words
catalase / B acillus subtilis / plasmid vector expression / ferm entation optimization引用本文复制引用
周丽萍,张东旭,李江华,堵国成,陈坚..过氧化氢酶基因在大肠杆菌中的克隆表达及发酵优化[J].工业微生物,2011,41(3):66-70,5.基金项目
国家自然科学基金重点项目(20836003) （20836003）
973项目(2007CB714306) （2007CB714306）
教育部新世纪优秀人才支持计划(NCET-07-0378). （NCET-07-0378）
