安徽医科大学学报2011，Vol.46Issue(5)：443-446,4.
新型维甲酸衍生物体外诱导白血病细胞分化
Effects of the retinoic acid derivatives on differentiation of leukemia cell in vitro
洪凡青 1陈飞虎 1王璐 1陈慧慧 1吴菲 1吴繁荣1
作者信息
摘要
Abstract
Objective To study the effect of differentiation induced by 4-amino-2-trifluoromethyl-phenyl acitretinate( ATPA ) on human leukemia NB4 cell. Methods NB4 cells were cultured in vitro and treated with ATPA of different concentration. The proliferation was studied by MTT test and growth curve of cell. Nitroblue tetrazolium ( NBT ) reduction test was used to analyze cell differentiation indexs. The cell cycle and the expression of the maturation specific cell surface markers were analyzed by flow cytometry( FCM ). Results ATPA had powerful activity in inbibiting proliferation and inducing differentiation. After treated with ATPA, the growth of leukemic cells was inhibited in a dose-dependent and time-dependent manner. The NBT positive cell ratio increased which indicated that ATPA could induce differentiation of NB4 cells. The expression level of the maturation specific cell surface marker CD11b of NB4 cells were increased while the expression level of CD13 was decreased. The proportion of cells in G0/G1 phase increased while S phase cells decreased. Cell cycle progression was blocked in the G1 phase. Conclusion ATPA can induce differentiation of leukemia cell NB4.关键词
维甲酸/类似物和衍生物/白血病/细胞分化Key words
retinoin/analogs & derivatives/ leukemia/ cell differentiation分类
医药卫生引用本文复制引用
洪凡青,陈飞虎,王璐,陈慧慧,吴菲,吴繁荣..新型维甲酸衍生物体外诱导白血病细胞分化[J].安徽医科大学学报,2011,46(5):443-446,4.
