畜牧兽医学报2011，Vol.42Issue(4)：475-480,6.
马身猪和大白猪不同组织DECR1基因的表达
Expression Analysis of DECR1 Gene in Tissues of Mashen and Large White Pig
摘要
Abstract
The purpose of this study was to analyze DECR1 mRNA and protein expression profiles of Mashen and Large White pigs in different tissues, and evaluate the potential relationships between its relative expression and lipid metabolism. DECRI expression profiles in nine tissues (liver, kidney, spleen, lung, small intestine, subcutaneous adipose, stomach, longissimus dorsi,heart) of Mashen and Large White pigs were examined by quantitative real-time PCR and Western blot, respectively. The results indicated that, by real-time PCR, DECR1 had the tissu- and species-specific in mRNA expression, but highly in liver, subcutaneous adipose and heart. The DECR1 mRNA expression in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig (P＜0. 01). The results showed that, by Western blot, the distribution of DECR1 in tissues were significantly different, but highly in subcutaneous adipose, liver and small intestine. Moreover, the expression of DECR1 in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig (P＜0. 05). It was inferred that DECR1 gene may be related to regulatory mechanism of lipid metabolism.关键词
猪/DECR1基因/实时荧光定量PCR/Western blot/组织表达分类
农业科技引用本文复制引用
李步高,郭晓红,曹果清,王效京,高鹏飞,刘宏,周忠孝..马身猪和大白猪不同组织DECR1基因的表达[J].畜牧兽医学报,2011,42(4):475-480,6.基金项目
山西省科技攻关项目(021043,20080311031) （021043,20080311031）
国家农业科技成果转化项目(2008GB2A300032) （2008GB2A300032）
