畜牧兽医学报2011,Vol.42Issue(4):533-537,5.
3种禽类呼肠孤病毒血清学相关性及致细胞病变差异分析
The Comparison of Serologic Relativity and CPE Types of 3 Avian Reovirus Strains Induced Different Diseases
摘要
Abstract
This study was conducted to compare the antigenic relativity and cytopathic effect (CPE) characteristics of 3 avian reovirus isolates induced different diseases. The serologic relatedness of avian reovirus (ARV) S1133 strain, muscovyduck reovirus (MDRV) MW9710 strain,novel duck reovirus (NDRV) NP01 strain were determined using cross virus -neutralization (VN)tests. Two avian and five mammalian cell lines were evaluated for their application in propagating these avian reovirus strains. Relatedness (R) values, determined by cross VN, revealed that three isolates' antigen had major differences. These strains could be cultivated successfully in Vero, MDEK, CEF, MDEF, ST, AD293 T cell lines and make some different types of CPE to different cell lines. These results indicated that ARV, MDRV and NDRV' antigen had major differences and did not share same antigenic relationship with each other. Avain reovirus of different disease-type could be distinguished by the types of CPE.关键词
禽呼肠孤病毒/番鸭呼肠孤病毒/新型鸭呼肠孤病毒/中和试验/抗原性变异/细胞病变分类
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陈仕龙,陈少莺,程晓霞,江斌,林锋强,王劭,朱小丽,李兆龙,张世忠..3种禽类呼肠孤病毒血清学相关性及致细胞病变差异分析[J].畜牧兽医学报,2011,42(4):533-537,5.基金项目
福建省公益类项目(2010R1101003-5) (2010R1101003-5)
福建省农科院创新团队项目(STIF-Y02) (STIF-Y02)