畜牧兽医学报2011，Vol.42Issue(4)：600-604,5.
猪链球菌对氟喹诺酮类药物的耐药性与靶位突变相关性
Relationship between Fluoroquinolone Resistance and Gene Mutation in streptococcus suis
摘要
Abstract
To detect the relationship between fluoroquinolone resistance and gyrA and parC gene mutation in Streptococcus suis. The MIC of 34 strains of Streptococcus suis were determined by microdilution. The genes encoding the quinolone-resistance determining region (QRDRs) of parC and gyrA in fluoroquinolone-susceptible and -resistant Streptococcus suis clinical isolates were identified and sequenced. Ser to Phe and Arg to Leu mutation at position 79 and 87 of the parC gene was detected in fluoroquinolone resistance S. suis, and Arg to Ser, or Ser to Arg mutations at the position 66 or 81 of gyrA gene were detected in 4 highly resistance strains; no amino acid changes in gyrA or parC were detected for 9 fluoroquinolone-susceptible strains, the mutations in both genes was found in the strains with MIC of fluoroquinolone higher than 32μg · L-1. Mutations in parC gene result in low level resistance against fluoroquinolone, and high level resistance is resulted from the mutations in gyrA and parC both genes.关键词
猪链球菌/氟喹诺酮类药物/parC基因/gyrA基因分类
农业科技引用本文复制引用
芮萍,马增军,段玲欣,刘谢荣,倪静,沈建忠..猪链球菌对氟喹诺酮类药物的耐药性与靶位突变相关性[J].畜牧兽医学报,2011,42(4):600-604,5.基金项目
河北省自然基金资助项目(C2009000877) （C2009000877）
河北省科技厅科技支撑项目(09220402D) （09220402D）
河北省教育厅计划项目(2008448) （2008448）
