浙江农林大学学报2011，Vol.28Issue(3)：349-358,10.
土大黄苷与牛血清白蛋白结合反应机制及金属离子的介导作用
Binding interactions between rhaponticin and bovine serum albumin with mediation of metal ions
摘要
Abstract
The interaction between rhaponticin (RT) and bovine serum albumin (BSA) was studied using fluorescence spectroscopy and absorbance spectra. Based on different theoretical models of fluorescence quenching, the binding constant (K) and binding sites (n) of the interactions were determined with the effects of metal ions on the interactions considered. The binding distance (r) and energy-transfer efficiency (E) between RT/RT-Co ( Ⅱ )/RT-Ni ( Ⅱ ) and BSA were also obtained using the Forster theory of non-radiation energy transfer.Results showed that RT bound BSA by forming a stable complex and the micro-conformation of BSA changed greatly due to a hydrophobic change in the chemical environment of the fluorescence chromophores within the relevant subdomains of BSA. Therefore, metal ions Co ( Ⅱ ) and Ni ( Ⅱ ) could compete with RT-BSA interactions, thereby shortening the binding distance between RT and BSA; however, to precisely quantify the hydrophobic effect and micro-conformational changes of drugs on proteins, we suggest a new formula defining the entention degree ED.关键词
土大黄苷/牛血清白蛋白/荧光光谱/紫外光谱:金属离子分类
农业科技引用本文复制引用
徐兴涛,郭明,吴志武,李铭慧,高兴军,钱慈..土大黄苷与牛血清白蛋白结合反应机制及金属离子的介导作用[J].浙江农林大学学报,2011,28(3):349-358,10.基金项目
国家自然科学基金资助项目(20877072) （20877072）
