安徽农业大学学报2011，Vol.38Issue(2)：151-155,5.DOI:CNKI:34-1162/S.20110303.1013.018
野葛异黄酮糖苷的分离纯化及体外清除自由基活性的研究
Free radical scavenging capacities of isoflavonoid glycosides isolated from Pueraria Radix
摘要
Abstract
Crude total isoflavonoids extracted from Pueraria Radix were partitioned by different organic solvents, then isolated and purified by several column chromatographic techniques and four isoflavonoid glycosides were obtained. On basis of the NMR and ESI-MS spectra, these four compounds can be identified as puerarin, daidzin, ononin, and genistin, respectively. Using an organic free radical system of 1, 1-diphenyl-2-picrylhydrazyl (DPPH · ) and an inorganic free radical system of superoxide anion free radical, the free radical scavenging capacities and antioxidant activities of these four isoflavonoid glycosides were examined in vitro. The results showed that all the four isoflavonoid glycosides were discovered to possess the scavenging capacities of the DPPH · free radical and superoxide anion radical. Meanwhile, there was a significantly positive correlation between the two free radical scavenging capacities and the treated concentrations of the four isoflavonoid glycosides. Genistin showed the highest DPPH · free radical scavenging capacity, followed by daidzin and puerarin,and ononin was the lowest among them. However, the superoxide anion free radical scavenging capacities of the four isoflavonoid glycosides were different from the former. Puerarin was discovered to be the strongest among them, followed by daidzin, genistin and ononin.关键词
异黄酮糖苷/分离纯化/清除自由基/野葛Key words
isoflavonoid glycoside/ isolation and purification/ free radical scavenging capacity/ Pueraria lobata (Willd) Ohwi.分类
农业科技引用本文复制引用
杨华,韩坤,宛晓春,方从兵..野葛异黄酮糖苷的分离纯化及体外清除自由基活性的研究[J].安徽农业大学学报,2011,38(2):151-155,5.基金项目
国家自然科学基金(30500051和30770230)和安徽农业大学校长青年基金(2007nqr22)共同资助. （30500051和30770230）
