安徽农业大学学报2011，Vol.38Issue(2)：232-237,6.DOI:CNKI:34-1162/S.20110303.0953.016
兼抗玉米矮花叶病毒及粗缩病毒无选择标记siRNA复合表达载体的构建
Construction of marker-free siRNA complex expression vector against MDMV and MRDV
摘要
Abstract
Based on the coat protein gene sequence of MDMV and MRDV in GenBank and MaizeSeq database, six pairs of specific primers were designed and six specific fragments were amplified by RT-PCR to prepare siRNA that corresponded to part of the MDMV CP or MRDV CP gene. In the first cloning step, an inverted repea1 sequence of pUCCRNAi + 2 F was constructed. Next, two inverted repeat sequences were inserted into pBluserip1 SK in series to generate pBluscript + SM. Meanwhile, Ubiquitin promoter and Nos termination were cloned into pDTB to generate pDTBU. In the third step, pDTBU and pBluscript + SM plasmids were digested and joined to generate pDTBUSM. The study presented here provides a valuable tool for plant viral control using RNAi and the PTGS approach.关键词
玉米矮花叶病毒/玉米粗缩病毒/外壳蛋白/栽体构建/siRNAKey words
maize dwarf mosaic virus (MDMV)/ maize rough dwarf virus (MRDV)/ coat protein (CP)/ vector construction/ siRNA分类
农业科技引用本文复制引用
张姣,赵阳,甘德芳..兼抗玉米矮花叶病毒及粗缩病毒无选择标记siRNA复合表达载体的构建[J].安徽农业大学学报,2011,38(2):232-237,6.基金项目
国家高技术研究与发展计划(863)项目(2008AA102408)和安徽省高校省级科研项目(KJ2011A110)共同资助. （863）
