中国计量学院学报2011，Vol.22Issue(1)：73-79,7.
三聚氰胺人工抗原及多克隆抗体的制备
Preparation of artificial antigen of melamine and polyclonal antibody
摘要
Abstract
Through the method of glutaraldehyde, the melamine(MEL) was coupled to the carrier protein for synthesizing the artificial antigen. Melamine was linked to bovine serum ovabumin (BSA) to generate immunogenic antigen (MEL-BSA) and was linked to ovabumin (OVA) by melamino-formaldehyde resin method to generate coating antigen (MEL-OVA). Artificial antigen was purified by FPLC, and then identified by UV and IR as well as SDS-PAGE. Mel-BSA was used to immune New Zealand white rabbits, and the generation of antibody was detected. The results show that the titer of antiserum is 1: 20 000. The synthesis of MEL artificial antigen is be possible to establish a basis for the detection of melamine by immunoassay.关键词
三聚氰胺/人工抗原/多克隆抗体Key words
melamine/ artifical antigen/ polyclonal antibody引用本文复制引用
龚云飞,王唯芬,张明洲,陈宗伦,彭雪,李沐洁,奚茜..三聚氰胺人工抗原及多克隆抗体的制备[J].中国计量学院学报,2011,22(1):73-79,7.基金项目
浙江省重大科技专项(No.2006C12102),杭州市重大科技创新专项(No.20083212A25),浙江省大学生科技创新项目 (No.2009R409031) （No.2006C12102）
