湖南农业大学学报（自然科学版）2011，Vol.37Issue(2)：166-171,6.DOI:10.3724/SP.J.1238.2011.00166
4个品种芍药愈伤组织的诱导及分化
Callus induction and differentiation of four peony cultivars
摘要
Abstract
Stems, petioles and leaves of four peony cultivars called Dafugui, Taohuafeixue, Karl Rosenfield and Sarah Bernhardt were selected to study major factors affecting the induction and regeneration of peony callus. The best peony cultivars was Dafugui and Karl Rosenfield in callus induction. Stem had an better effect than petiole. The best medium for callus induction was WPM+2.0 mg/L 6-BA +0.2 mg/L NAA +0.2 mg/L 2,4-D. It was better to sample explants in stooling stage than in leaf-expansion period. The optimum medium for callus proliferation were 1.0 mg/L NAA +2.0 mg/L 6-BA +0.1 mg/L TDZ for Dafugui and 1.0 mg/L NAA +0.5 mg/L 6-BA +0.1 mg/L TDZ for Karl Rosenfield. It was hard to induce adventitious bud, only some white bud points can be seen. The optimum medium for adventitious root induction was WPM+0.5 mg/L IBA, and the rooting rate was 50.00％.关键词
芍药/愈伤组织/茎段/不定芽/不定根Key words
Paeonia lactiflora Pall./ stem/ callus induction/ adventitious bud/ adventitious roots分类
农业科技引用本文复制引用
于晓南,吴红娟,潘瞳..4个品种芍药愈伤组织的诱导及分化[J].湖南农业大学学报（自然科学版）,2011,37(2):166-171,6.基金项目
国家"十二·五"科技支撑项目(20113AD12B02) （20113AD12B02）
中央高校基本科研业务费专项(BLYX200931) （BLYX200931）
