广西植物2011，Vol.31Issue(2)：262-265,4.DOI:10.3969/j.issn.1000-3142.2011.03.023
睫毛萼凤仙花的组织培养及其愈伤组织的超微观察
Tissue culture of Impatiens blepharosepala and ultrastructural observation of callus
摘要
Abstract
Leaves, stem and root of Impatiens blepharosepala seedling were cultured in vitro, and two types of callus having different differentiation were observed by electron microscope. The results showed that the induction rate was highest on MS+NAA 0. 5 mg/L+6-BA 0. 5 mg/L,the induction rate of leaves was 100％ and the rate of stems was 67％ ,but the roots can't be induced. Callus of leaves can be induced buds on MS+NAA 0.5 mg/L+6-BA 2.0 mg/L after 40 d,but there was no organ generated from stems. Cells of callus having differentiation arrayed irregularly and there was obviously similar tissue differentiation by electron microscope.关键词
睫毛萼凤仙花/离体培养/超微观察Key words
Impatiens blepharosepala/ culture in vitro/ ultrastructural observation分类
生物科学引用本文复制引用
王雪娟,程新利,柴瑞娟..睫毛萼凤仙花的组织培养及其愈伤组织的超微观察[J].广西植物,2011,31(2):262-265,4.基金项目
安徽省自然科学基金(KJ2009B166Z,KJ2008B07ZC) （KJ2009B166Z,KJ2008B07ZC）
安徽省高校自然科学基金(KJ2009B123Z) （KJ2009B123Z）
安徽工程科技学院青年教师科研项目(2008RZR004) （2008RZR004）
