山东农业大学学报（自然科学版）2011，Vol.42Issue(2)：205-210,6.
黄牛白细胞介素-2基因的克隆与序列分析
CLONING AND SEQUENCE ANALYSIS ON IL- 2 GENE IN CATTLE
摘要
Abstract
In order to clone cattle interleukine - 2 gene, according to GenBank in the bovine Interleukine - 2 ( IL -2) cDNA sequences, a size of 500 bp cDNA fragment was amplified by RT -PCR from the total RNA extracted by ConA stimulated 12- hours cattle peripheral blood lymphocytes, and then constructed pMD- 18T- IL- 2 recombinant vector, following identification of PCR and double digestion of EcoR - 1 and BamHI.Sequence analysis indicated that cattle IL -2 gene catained an open reading frames (ORF) of 468 bp.Compared the nucleotide with bovine, buffalo, sheep, cat, horse, chicken, canin,human, goat, mice and pig, the homoloaies were 98.9％ ,98.5％ ,97.0％, 82.4％ ,79.9％ ,5.3％ ,78.2％ ,79.7％ ,95.9％ ,65.2％ and 84.7％ ,respectively.关键词
黄牛/IL-2/克隆/序列分析Key words
Cattle/ IL - 2/ cloning/ sequence analysis分类
农业科技引用本文复制引用
刘杰,谢昆,蒋成砚,吴晶萍,韩雪,王冉..黄牛白细胞介素-2基因的克隆与序列分析[J].山东农业大学学报（自然科学版）,2011,42(2):205-210,6.基金项目
红河学院生物化学与分子生物学重点学科建设项目(071010) （071010）
红河学院大学生科技创新项目(SSTIF08044) （SSTIF08044）
