中国蔬菜Issue(6)：15-21,7.
马铃薯Desiree遗传转化体系的优化及转基因植株的获得
Optimization of Genetic Transformation System for Potato Variety 'Desiree' and Obtainment of Transgenic Lines
摘要
Abstract
The aim of this work was to optimize the transformation system for potato ( Solanum tuberosum L.) variety ' Desiree' and to obtain transgenic potato.Explants derived from potato leaves and stems were used as receptor materials to compare transformation efficiency with different time of pre-culture, Agrobacterium tumefaciens content, duration of infection and the proportion of hormones.The results showed that stem was most suitable for the induction of callus and shoots.Stem explants were pre-cultured for 2 days before infection by A.tumefaciens strain Agl-0 ( OD600=0.5, 10 min ) and selected on MS20 containing 1.0 mg· L-1 zeatin-trans ( ZT-t ) , the highest transformation efficiency was obtained with 80 ％ of callus induction and 70 ％ of differentiation.R 1, the gene resistant to potato late blight, was introduced into potato through above improved transformation system.PCR analysis to the kanamyein-resistant transformants revealed that R1 gene was integrated into potato genome.Transfer of R1 gene into potatoes had not any impact on agronomic traits compared to the non-transgenic line ( CK ) and the most transgenie lines had strong resistance to potato late blight verified by inoculation with isolate 89148-9 ( 0 ).关键词
马铃薯/遗传转化/外植体Key words
Potato/ Genetic transformation/ Explants分类
农业科技引用本文复制引用
辛翠花,郭江波,黄三文,屈冬玉..马铃薯Desiree遗传转化体系的优化及转基因植株的获得[J].中国蔬菜,2011,(6):15-21,7.基金项目
国家自然科学基金项目(30671319),中荷园艺作物基因组分析联合实验室运行项目(1251601001),国家重点基础研究发展计划项目(2006cb101907),中荷战略联盟项目(2004CB720405) （30671319）
