中国蔬菜Issue(6)：49-53,5.
基于5.8SrDNA和ITS序列探讨亚洲瓠瓜的地理分化
Studiesy on Geographic Divergence of Lagenaria siceraria ssp.asiatica Based on 5.8S rDNA and ITS Sequence Data
摘要
Abstract
The geographic divergence of the Lagenaria siceraria ssp. asiatica were examined using ITS1+5.8S rDNA+ITS2 sequences data. The sequences were obtained from 23 species of L. siceraria ssp. asiatica from China, Thailand and Japan. The results showed that within L. siceraria ssp. asiatica, ITS1+5.8S rDNA+ITS2 was 611-627 bp, the G+C content of the sequences was 54.17 ％-63.26 ％, 65 informative sites ( 11.09 ％ ) within L. siceraria ssp. asiatica are found in the ITS1+5.8S rDNA+ITS2 sequences. The length of the ITS1+5.8S rDNA+ITS2 sequences of 2 species from Japan is shorter than the other 21 species from China and Thailand, the G+C content of the sequences of the 2 species from Japan is lower than those from China and Thailand. Finally we constructed the strict consensus tree of 23 species of L. siceraria ssp. asiatica indicated that L. siceraria ssp. asiatica has two main prophylactic glades, one including 2 species from Japan, and the other including 20 species from China and 1 species from Thailand. The bootstrap value is 99 ％. Test of the homogeneity of substitution patterns revealed that the species of L. siceraria ssp. asiatica from Japan are significantly different from those from China and Thailand.关键词
5.8S rDNA/亚洲瓠瓜/内转录间隔区/地理分化Key words
5.8S rDNA/ Lagenaria siceraria ssp. asiatica/ ITS/ Geographic divergence分类
农业科技引用本文复制引用
周先治,陈阳,陈晟,吴宇芬,赵依杰..基于5.8SrDNA和ITS序列探讨亚洲瓠瓜的地理分化[J].中国蔬菜,2011,(6):49-53,5.基金项目
福建省科技厅重点项目(2007I0037) （2007I0037）
