中国畜牧兽医2011，Vol.38Issue(3)：155-159,5.
猪分级卵母细胞体外成熟时间规律的研究
Research of Pig Oocytes in vitro Maturation Time
摘要
Abstract
The objectives of the present study were first to carry out the cumulus-oocyte complexes (COCs) divided into levels A, B, C and D according to cumulus cells, which levels A and B were cultured in vitro for 24, 32, 38, 44, 48 and 54 h,and C was cultured in vitro for 24, 32, 38, 44, 48, 54 and 60 h, respectively.Subsequently, the oocytes with extruded polar body at different time intervals were induced by application of an AC pulse of 10 V for 5 s followed by 1.6 kV/cm for 60 μs using an Electro Cell Manipulator 2001.These results showed that the maturation rate of COCs of levels A and B arrived at the highest period was no significant difference (83.2％ and 78.0％, P＞0.05) in vitro for 48 h, but both were significantly higher than the same level COCs cultured for 24, 32 and 38 h.Different levels of COCs culture same time in vitro, the maturation rate of levels A and B were not significant (P＞0.05), but both were significantly higher than the levels C.The rate of cleavage (78.2％) in vitro culture for 44 h was significantly higher(P＜0.05) than COCs cultured for 24 to 38 h.In conclusion, COCs of levels A and B were more suitable for oocytes cultured in vitro, obtaining a high maturation rate and cleavage, granule cells had significant influence on oocyte maturation.COCs cultured in vitro before 38 h, some oocytes may be observed the polar body, but the developmental rate to cleavage were extremely lower, indicating that oocytes were no really mature.At the same time,oocytes of the developmental maturation could not judge only by excluding the polar body.Oocytes cultured in vitro 44 to 48 h was the best period of in vitro maturation(IVM) and provide a number of high-quality metaphase-Ⅱ (M Ⅱ ) oocytes for production of parthenotes and somatic cell nuclear transfer(SCNT) embryos.关键词
卵丘-卵母细胞复合体/体外成熟/极体/孤雌激活Key words
COCs / IVM/ polar body / parthenogenetic activation分类
农业科技引用本文复制引用
华再东,郑新民,魏庆信,许厚强,乔宪凤,刘西梅,李莉,肖红卫,王亚刚..猪分级卵母细胞体外成熟时间规律的研究[J].中国畜牧兽医,2011,38(3):155-159,5.基金项目
国家生物新品种培育专项(2008ZX08006-003 （）
2007ZX08006-002 （）
2007ZX08010-003 （）
2008ZX08011004 （）
2009ZX011-030B) （）
国际合作(2009BFA012). （2009BFA012）
