安徽农业科学2011，Vol.39Issue(9)：5245-5246,2.
黄鳝抗菌肽hepcidin基因大肠杆菌表达载体的构建
Constructing of A E. coli Expression Vector of Anti-bacterial Peptides Hepcidin Gene from Eel
摘要
Abstract
[ Objective ] To construct a E. coli expression vector of hepcidin gene from rice field eel. [ Method ] According to hepcidin gene sequence of rice field eel reported by genbank, primers were designed , and PCR was performed to amplify the hepcidin gene. Then the purified fragment was cloned into pET-28a vector. [ Result] Hepcidin gene was amplified from the cDNA of rice field eel. The gene fragment was 291 bp, and was cloned into pET-28a vector. After analysis of restriction endonuclease digestion and PCR, the constructed expression vector was proved to be correct. [ Conclusion ] The expression vector of hepcidin gene of rice field eel was constructed successfully.关键词
黄鳝/抗菌肽/基因/原核表达载体Key words
Rice field eel/ Anti-bacterial peptides/ Hepcidin Gene/ E. coli expression vector分类
农业科技引用本文复制引用
刘成都,邱庆崇,耿晓雪,李伟..黄鳝抗菌肽hepcidin基因大肠杆菌表达载体的构建[J].安徽农业科学,2011,39(9):5245-5246,2.基金项目
长江大学2009年大学生创新性实验计划项目(0901). （0901）
