安徽农业科学2011，Vol.39Issue(9)：5308-5309,5514,3.
甘蔗脱毒健康种苗宿根矮化病的PCR检测
Detection of Ratoon Stunting Disease in Virus-free Seedcane of Saccharum officinarum by PCR
摘要
Abstract
[ Objective ] The study was carried out for providing good method to detect ratoon stunting disease (RSD) in virus-free seedcane of Saccharum officinarum. [ Method] PCR method was used for the detection of RSD in virus-free seedcane of sugarcane. [ Result] During the different growth stages of healthy virus-free seedling including proliferation stage, rooting stage, sand culture stage and temporary planting stage, the RSD detection results were negative. PCR detection sensitivity in the present study showed that PCR could detect RSD pathogen in the solution with bacterium concentration 10-3. [ Conclusion ] PCR method showed a good specificity and higher sensitivity, so it was suitable for the batch detection in the production of healthy virus-free seedlings of sugarcane.关键词
甘蔗/健康种苗/甘蔗宿根矮化病/PCRKey words
Sugarcane/ Virus-free seedcane/ Ratoon stunting disease/ PGR分类
农业科技引用本文复制引用
淡明,李松,余坤兴,刘丽敏,刘红坚,戴友铭..甘蔗脱毒健康种苗宿根矮化病的PCR检测[J].安徽农业科学,2011,39(9):5308-5309,5514,3.基金项目
广西甘蔗研究所基本科研业务专项(G2009006). （G2009006）
