安徽农业科学2011，Vol.39Issue(9)：5259-5260,5262,3.
猪饲料用乳酸菌增菌培养基的优化研究
Optimization of Lactic Acid Bacteria Culture Conditions
摘要
Abstract
[ Objective ] The optimum enrichment medium of lactic-acid bacteria was optimized for the lactic-acid bacteria inoculants production of pig feed preparation by the study of pH and medium component. [ Method ] The optimum pH of medium was determined with pH gradient culture. The main effect factors were determined with Plackett-Burrman (PB) design. The optimum enrichment medium and the interrelationship were achieved through Response Surface Methodology ( RSM ). [ Result ] The results showed that the optimum pH6.8, the main effectfactors were beef extract, glucose and NaAe; the most important factor was beef extract; the optimum enrichment medium was beef extract 23.70 g/L, glucose 47.53 g/L, NaAe 4.87 g/L, Yeast extract 10. 0 g/L, diammonium citrate 2. 0 g/L, MnSO4 0.2 g/L, Tween80 0. 5 g/L.[ Conclusion ] The optimized lactic acid bacteria culture condition made the density of live bacterial enhanced significantly and reached the needs of production of lactic acid bacterium.关键词
乳酸菌/增菌培养基/优化/菌体密度Key words
Lactic-acid bacteria/ Enrichment medium/ Medium optimization/ Cell density分类
农业科技引用本文复制引用
杜刚,田孟华,杨海英,杨胜江..猪饲料用乳酸菌增菌培养基的优化研究[J].安徽农业科学,2011,39(9):5259-5260,5262,3.基金项目
云南省教育厅科学研究基金项目(08Z0045) （08Z0045）
云南民族大学青年基金科研项目(09QN08). （09QN08）
