安徽农业科学2011，Vol.39Issue(9)：5263-5264,2.
一种适用于多数植物病原真菌的单孢分离方法
Research on the Isolation Method of Single Spore of Most Plant Pathogenic Fungi
摘要
Abstract
[ Objective ] The isolation technique of single spore, which was suitable for various pathogenic fungi, was researched. [ Method ] The isolation technique of single spore of Bipolaria maydis was improved and the single spore, which had germinated and scattered in the water agar plate, was picked up after three steps: tissue isolation, coating spore suspension and removal of dissecting needle. [ Result] The single spore of most of samples was isolated with the technique, which successful rate was over 92％ and partially 100％; the successful rate of the isolation of single hyphal fragment was over 60％. [ Conclusion] The experimental result showed that the method was easy to operation, the requirement to material and equipment was simple, the result was reliable, the successful rate was high and the approach of its applicability was wide.关键词
玉米小斑病/单孢分离/组织分离/挑针转移Key words
Bipolaria maydis/ Single spore isolation/ Tissue isolation/ Removal of dissection needle分类
农业科技引用本文复制引用
邱小燕,汤智鹏,张敏,荆海瑜..一种适用于多数植物病原真菌的单孢分离方法[J].安徽农业科学,2011,39(9):5263-5264,2.基金项目
国家玉米产业技术体系项目. （）
