果树学报2011，Vol.28Issue(3)：463-468,后插2,7.
我国葡萄主栽区卷叶病相关病毒种类的检测分析
Study of Grapevine leafroll-associated viruses in China
摘要
Abstract
Two hundred grapevine cultivars were surveyed for the prevalence of grapevine leafroll disease(GLD). 82 cultivars showed GLS symptoms, and the cultivars belong to Vitis vinifera L. had more cultivars, higher morbidity and more serious symptoms than V. vinifera-V. labrusca. No V. labrusca L. was found showing GLD symptoms in the vineyards. Fifty eight samples collected from the vineyards were tested for GLRaVs by ELISA and RT-PCR. The positive rate of GLRaV-1, 2, 3, 4, 5 and 7 was 20.7％,17.2％, 62.1％, 3.4％, 5.2％ and 15.5％ respectively. The recovered fragments were cloned, sequenced and identified. The sequencing results have been submitted to GenBank. In this paper, the evolutionary relationship of GLRaVs was conducted based on HSP70 encoding genes. Neighbor-Joining (NJ)phylogenetic trees was established, and GLRaV-1, 3 and 7, GLRaV-2, 4 and 5 were classified together in a cluster respectively. Moreover, GLRaV-1 and 3 have closer genetic relation than GLRaV-7. And GLRaV-4 and 5 have closer genetic relation than GLRaV-2.关键词
葡萄卷叶病毒/田间调查/ELISA/RT-PCR/进化树Key words
Grapevine leafroll-associated viruses (GLRaVs)/ Investigation of occurrence/ ELISA/ RTPCR / Phylogenetic分类
农业科技引用本文复制引用
裴光前,董雅凤,张尊平,范旭东,任芳..我国葡萄主栽区卷叶病相关病毒种类的检测分析[J].果树学报,2011,28(3):463-468,后插2,7.基金项目
国家葡萄产业技术体系建设项目(编号:nycytx-30-bc-02) （编号:nycytx-30-bc-02）
