果树学报2011，Vol.28Issue(3)：469-473,后插1,6.
北京地区核桃黑斑病病原菌的分离、致病性测定和16S rDNA序列分析
Identification and pathogenicity assay of the pathogen of walnut blight in Beijing
摘要
Abstract
Walnut blight is a severe disease on walnut production. In 2008, a suspected blight disease was found in walnut trees planted in rural area of Beijing. In order to confirm the taxonomic status of the pathogen, bacteria were isolated and purified from plant samples with typical symptoms. Pathogenicity was confirmed by artificial inoculation of healthy walnut branches and leaves with a suspension of an isolated bacterial strain. A 1.5 kb of 16S rDNA fragment amplified by PCR using bacterial 16S rDNA universal primers was cloned into pMD-18T vector. Sequencing analysis revealed that the fragment contains 1453 nueleotides which is highly similar to the 16S rDNA of bacterial pathogen Xanthomonas eampestris.关键词
核桃黑腐病/细菌/致病变种Key words
Walnut blight/ Bacteria/ Pathovar分类
农业科技引用本文复制引用
陈善义,陶万强,王合,李金钟,周涛..北京地区核桃黑斑病病原菌的分离、致病性测定和16S rDNA序列分析[J].果树学报,2011,28(3):469-473,后插1,6.基金项目
中央高校基本科研业务费专项资金(2009-1-58) （2009-1-58）
