果树学报2011，Vol.28Issue(3)：531-535,5.
测定葡萄与葡萄酒总酚的二次回归正交旋转优化设计
Optimizing a simple determination method for total phenolics in grape and wine through quadratic regression rotational combination design
摘要
Abstract
A simple method to determine total phenolic compounds in grape and wine was established. The reaction conditions were optimized through quadratic regression rotational combination design basing on the single factor experiments such as Na2CO3 concentration, reaction temperature and reaction time using pipette. The orthogonal experimental results showed that the optimum reaction system including 0.5 mL sample, 0.5 mL 1 mol· L-1 Folin-Ciocalteu reagent and 4.0 mL 52 g· L-1 Na2CO3 incubated at 25 ℃ for 102 min, then determined the extinction value at 765 nm. The calibration curve showed a good linearity between the absorbance value and gallic acid concentration range from 0 g· L-1 to 0.050 g· L-1 (R2=0.999 4). The average recovery rate evaluated by testing the berry of Cabernet Sauvignon and wine at three concentration (low, middle and high) were 98.4％ and 97.8％ (n=3), respectively, with the corresponding relative standard deviation (RSD) of 1.32％ and 2.01％. It's a simple and economic method for phenolic compound determination in the grape and wine with high stability and accuracy.关键词
葡萄/葡萄酒/总酚/Folin-Ciocalteu法/二次回归正交旋转组合设计Key words
Grape/ Wine/ Total polyphenols/ Folin-Ciocalteu assay/ Quadratic regression rotational combination design分类
农业科技引用本文复制引用
徐国前,栾丽英,焦旭亮,张振文..测定葡萄与葡萄酒总酚的二次回归正交旋转优化设计[J].果树学报,2011,28(3):531-535,5.基金项目
现代农业产业技术体系建设专项资金(nycytx-30-zp-04) （nycytx-30-zp-04）
