热带作物学报2011，Vol.32Issue(1)：89-93,5.DOI:10.3969/j.issn.1000-2561.2011.01.019
海马齿胚胎发育晚期丰富蛋白基因SpLea5的克隆与表达分析
Cloning and Expression Analysis of a Late Embryogenesis Abundant Protein Gene SpLea5 from Sesuviumportulacastrum
摘要
Abstract
Based on the EST sequences from a differentially expressed eDNA library of Sesuvium portulacastrum roots, the full-length eDNA of a late embryogenesis abundant pnotein gene designated as SpLea5 was cloned from S. portulacastrum roots. Sequence analysis showed that the SpLea5 eDNA contained 685 bp with an open reading frame (ORF) of 294 bp and encoded 97 amino acid residues. The deduced amino acid sequence shared the identity of 54％, 52％, 48％, 47％, 47％,46％, 46％, 44％ and 41％ with that from Tamarix androssowii, Populus suaveolens, Nicotiana tabacum, Citrus sinensis,Citrus unshiu, Jatropha curcas, Gossypium hirsutum, Glycine max and Ricinus communis, respectively. In phylogenetic tree the SpLea5 and other plant-derived Leas were grouped into one large cluster. Semi-quantitative RT-PCR analysis revealed that the SPLea5 was constitutively expressed in all tested organs but at different levels. The SpLea5 was strongly expressed in roots, but weakly in leaves and stems. The expression of SpLea5 was strongly regulated by seawater, NaCI, PEG and ABA, suggesting that the SpLea5 in S.portulacastrum may be involved in responses to salt stress and drought.关键词
海马齿/胚胎发育晚期丰富蛋白基因/表达分析Key words
S.portulacastrum/ late Embryogenesis Abundant Protein gene/ Expression profile分类
生物科学引用本文复制引用
付桂,范伟,畅文军,张治礼..海马齿胚胎发育晚期丰富蛋白基因SpLea5的克隆与表达分析[J].热带作物学报,2011,32(1):89-93,5.基金项目
中国热带农业科学院中央级公益件科研院所基本科研业务费专项(ITBBZD0716). （ITBBZD0716）
