高校地质学报2011，Vol.17Issue(1)：59-65,7.
分离自铜矿山尾矿区的铁还原菌nju-T1菌株:菌种鉴定及其还原Fe3+的最适条件
A Study on the Ferric Reduction Microorganism nju-T1 Isolated from Shizishan Tailings, Tongling, Anhui Province
摘要
Abstract
A new iron reduction strain, named nju-T1, was isolated from acid mine drainage (AMD) and surface tailings samples from Yangchongshan tailings zone, Shizishan, Tongling, Anhui province, with FeTo selective medium and overlay culture technology. The morphological, physiological and biochemical research and phylogenetic analysis based on the 16S rRNA gene sequence showed that the strain belongs to Acidithiobacillus. It was found that nju-T1 could reduce Fe3+under aerobic conditions with plate and liquid cultural tests. The pH range for growth was pH 2.0-6.0, with optimum growth at pH 4.5, optimum growth temperature at 30 C and optimum ferric iron concentration at 5 mmol/L. It was inferred that the reduction sites may be located on the cell outer membrane. AMD and tailings were controlled by the dynamic balance between the microbial functional groups and surroundings. The nju-T1 reduced ferric to ferrous, which provided initial energy to ferrous-oxidizing bacteria and maintained the Fe recycle and stability of acid mine environment. The research on mechanism of ferric reduction with nju-T1 not only broadens the knowledge about ferric reduction bacteria mainly growing under the anaerobic condition, but also has great significance in understanding the formation of acid mine environment, its development mechanism and its further environmental treatment.关键词
酸矿水/尾矿/Acidithiobacillus sp.nju-T1/系统进化树分析/三价铁还原Key words
acid mine drainage/ tailings/ Acidithiobacillus sp. nju-T1/ phylogenetic tree/ ferric reduction Corresponding author: Wang Ruiyong, Associate Professor. E-mail: wangry@nju.edu.cn分类
生物科学引用本文复制引用
邢辉,赵婷婷,许泽安,管思琪,陆现彩,陆建军,王睿勇..分离自铜矿山尾矿区的铁还原菌nju-T1菌株:菌种鉴定及其还原Fe3+的最适条件[J].高校地质学报,2011,17(1):59-65,7.基金项目
国家重点基础研究发展计划资助(2007CB815603)和国家自然科学基金重点项目(40930742)资助 （2007CB815603）
