果树学报2011，Vol.28Issue(3)：406-412,7.
ISSR标记在中国野生葡萄分类中的应用
Application of ISSR markers in taxonomy of Chinese wild grape
摘要
Abstract
16 Chinese wild grape species and their relatives were studied by ISSR markers on taxonomy.13 primers selected from 100 primers were used for ISSR amplification. 185 bands were generated which molecular weight ranged from 300 to 2 000 bp, of which 175 bands were polymorphic bands (the percentage of polymorphic band, PPB＝94.6％). According to the results of ISSR amplification, the similarity coefficient were analyzed by NTSYSpc2.10e software, the genetic similarity coefficient was 0.57 to 0.99. The clustering dendrogram was constructed by UPGMA method to explore the taxonomic relationship between 81 Vitis materials. To employ Ampelopsis sinica (Miq.) W. T. Wang. and Vitis rotundifolia as Outgroup,which had genetic similarity coefficient 0.57 and 0.60 with Euvitis, respectively. The 79 Euvitis materials were classified as 22 groups according to the cluster analysis of the PCR amplified products. Most of materials had the same traditional classification but some not. According to the results of ISSR clustering ,we determined the Maoputao 1099 as Vitis ftcifolia Bunge. Whether Yanshanputao 0947 is Vitis yeshanensis J. X. Chen was suspected. We also found inter-specie types existed in 10 Vitis piasezkii Maxim. Materials.关键词
中国野生葡萄/ISSR/遗传相似系数/分类Key words
Chinese wild grape/ ISSR/ Genetic similarity coefficient/ Taxonomy分类
农业科技引用本文复制引用
张永辉,刘崇怀,樊秀彩,张颖,孙海生,彭斌,姜建福..ISSR标记在中国野生葡萄分类中的应用[J].果树学报,2011,28(3):406-412,7.基金项目
现代农业产业技术体系建设专项资金(CARS-30) （CARS-30）
