果树学报2011，Vol.28Issue(3)：423-427,5.
梅(Prunus mume)IRAP分子标记技术体系的建立
A technique system of IRAP marker on mei(Prunus mume)
摘要
Abstract
Based on the Tyl-copia retrotransposons LTR sequences of Mei, primers for IRAP were designed. Studied on five factors L16 (45), four levels complete random orthogonal test, the various PAGE concentrations, etc., the final optimal system of IRAP marker technique is established. The results showed that the optimal system for IRAP was total in 25 μL, composed of template DNA 30 ng, 25 mmol.L-1 MgC12 2.0 μL, 2.5 mmol·L-1 dNTPs 2.5μL, 10×PCR Buffer 2.5 μL, Taq DNA polymerase 1.0 U, 10 μmol·L-1 LTR primer 1.0 μL, and ddH2O added to 25 μL. PAGE with the concentration of 6.0％ and 8.0％ non-denaturing polyacrylamide gel could present genetic diversity.关键词
梅/IRAP/技术体系Key words
Prunus mume Sieb. et Zucc./ IRAP/ Technique system分类
农业科技引用本文复制引用
沈玉英,高志红,王飞,佟兆国,房伟民,章镇..梅(Prunus mume)IRAP分子标记技术体系的建立[J].果树学报,2011,28(3):423-427,5.基金项目
国家自然科学基金(30070530) （30070530）
农业部公益性行业(农业)科研专项(201003058) （农业）
