山西农业大学学报（自然科学版）2011，Vol.31Issue(2)：156-160,5.
绵羊线粒体DNA D-loop区遗传多样性分析
Genetic Diversity Analysis of mtDNA D-loop in Sheep Breeds
摘要
Abstract
Genetic diversity of 5'-terminal partial sequence in mitochondrial DNA D-loop of 806 animals from eight sheep populations was studied by single strand conformation polymorphism (SSCP) technique based on polymerase chain reaction (PCR-SSCP). The emphasis was focused on the genetic differences between three hreeds (lines) originated in Shanxi Province (Shanxi Meat-type Dam Line, Shanxi Fine-wooled sheep and Jinzhong sheep) and two Chinese local and three introduced foreign breeds. Six haplotypes were found and sequencially designated as A,B,C,D,E and F. The primary haplotype was B for Dorper,E for Large-tailed Han, and F for other populations, respectlvely. The lowerest haplotype diversity was found in Corriedale (0. 109)showing the poor genetic variation. Much higher genetic variation,from 0. 579 for haplotype diversity of Shanxi Meat-type Dam Line to 0. 741 for those of Dorper, existed in other seven populations. The haplorype diversity of Shanxi Fine-wooled sheep and Jinzhong sheep were 0. 724 and 0. 624, respectively. Principal component analysis revealed close genetic relationship between Shanxi Meat-type Dam Line and Smalltailed Han and farther relationship among the other six breeds.关键词
绵羊/线粒体 DNA/PCR-SSCP/遗传多样性Key words
Sheep / Mitochondrial DNA / PCR-SSCP/ Genetic diversity分类
农业科技引用本文复制引用
林婄婄,高中元,武建亮,李建斌,乔利英,刘文忠..绵羊线粒体DNA D-loop区遗传多样性分析[J].山西农业大学学报（自然科学版）,2011,31(2):156-160,5.基金项目
国家自然科学基金(30972084) （30972084）
"十一五"国家科技支撑计划项目(2008BADB2B04-8) （2008BADB2B04-8）
山西省科技攻关项目(011029) （011029）
