分析化学2011，Vol.39Issue(4)：443-448,6.DOI:10.3724/SP.J.1096.2011.00443
基于丝网印刷碳电极的微囊藻毒素-(亮氨酸-精氨酸)的电流型免疫传感器
An Amperometric lmmunosensor for Microcystin-( Leucine-Arginine) Based on Screen-Printed Carbon Electrode
摘要
Abstract
An amperometric immunosensor for the detection of microcystin-(leucine-arginine)(MCLR) was prepared by immobilizing an MCLR-ovalbumin (MCLR-OVA) conjugate to an osmium bipyridyl complex-attached poly(4-vinylpyridine) membrane modified onto a screen-printed carbon electrode. The immunosensor was based on the indirect competitive immunoassay format with the labeled agent of horseradish peroxidase-conjugated goat anti-mouse immunoglobulin-G antibody. The current response decreased proportionally with the concentration of MCLR in the range of 0. 43-10.72 μg/L with a detection limit of 0.17 μg/L. The mean recoveries of standard additions in three real water samples were in the range of 83％ to 121％, and the RSD were in the range of 3.6％ to 7.6％. Thus, the proposed method is a promising technique for the detection of MCLR in environmental monitoring.关键词
免疫传感器/微囊藻毒素-(精氨酸-亮氨酸)/锇氧化还原聚合物/丝网印刷碳电极Key words
Immunosensor/ Microcystin-(leucine-arginine)/ Osmium redox polymer/ Screen-printed carbon electrode引用本文复制引用
陈向强,何苗,施汉昌,蔡强..基于丝网印刷碳电极的微囊藻毒素-(亮氨酸-精氨酸)的电流型免疫传感器[J].分析化学,2011,39(4):443-448,6.基金项目
本文系国家高新技术发展计划(Nos.2007AA06Z419,2009AA06A417)资助项目 （Nos.2007AA06Z419,2009AA06A417）
