畜牧与兽医2011，Vol.43Issue(4)：15-18,4.
牛附红细胞体单管套式PCR检测方法的建立
Establishment of a single-tube nested PCR assay for Mycoplasma wenyonii
摘要
Abstract
To establish a single-tube nested PCR method for the detection of Mycoplasma wenyonii. two pairs of primers were designed according to the published sequence of the 16S ribosomal RNA gene of M. wenyonii from GenBank ( AF016546) . Sequencing analysis showed that the targeted gene fragment was 361 bp in length and 98％ identical to the published data. The nested PCR method was specific and there were no cross reactions with 7heilena sergenti . Neospora caninum , Bacterium burgeri and Streptococcus agalactiae. The sensitivity of the assay was 1. 22 fg/μL of DNA. Among the 66 blood samples from Yanhian region of Jilin Province, the detection rate was 28. 8％ by single-tube nested-PCR, higher than conventional PCR ( 21. 2％ ) and blood pressure tablets (12. 1％ ) . It suggested that the established method was an accurate, specific and sensitive diagnostic technique for the lahoratory detection of M. wenyonii.关键词
牛附红细胞体/16S rRNA/单管套式PCRKey words
M. wenyonii/ 16S rRNA/ single-tube nested PCR分类
农业科技引用本文复制引用
邢莹,郑秀红,贾立军,薛书江,张守发..牛附红细胞体单管套式PCR检测方法的建立[J].畜牧与兽医,2011,43(4):15-18,4.基金项目
国家自然科学基金项目(30860203) （30860203）
公益性行业(农业)科研专项项目(200903036-13). （农业）
